Journal: Dentistry Journal

Article Title: Investigation of the Molecular Profile of Granular Cell Tumours and Schwannomas of the Oral Cavity

doi: 10.3390/dj10030038

Figure Lengend Snippet: Summary of the antibodies used for immunohistochemistry of GCTs and schwannomas.

Article Snippet: GAP43 , Bio-Techne Canada, Oakville On, NB300-143 , Rabbit polyclonal , Regenerating neural tissues/growth cones, GAP43 intracellular growth protein/membrane protein , 1/5000.

Techniques: Immunohistochemistry, Cell Surface Receptor Assay, Membrane, Plasmid Preparation

Journal: Neuroscience letters

Article Title: Overexpression of miR-338-5p in exosomes derived from mesenchymal stromal cells provides neuroprotective effects by the Cnr1/Rap1/Akt pathway after spinal cord injury in rats.

doi: 10.1016/j.neulet.2021.136124

Figure Lengend Snippet: Fig. 3. The expression of miR-338-5p was lower in the rats with SCI than the control rats, and the miR- 338-5p-overexpressing exosomes provided neuro protective effects after SCI. (A) Expression of miR- 338-5p was assessed by qRT-PCR at 1, 4 and 7 days after acute spinal cord injury in rats. (B, C) Western blot analysis of NF-M, GAP43, MAG and GFAP levels following various treatments at day 4 post-SCI. Data are presented as the mean ± SD. n = 4 per group (A), n = 3 per group (C), #p < 0.05, ##p < 0.01, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After the membranes were blocked with 5% nonfat milk in TBST for 1 h at room temperature, they were incubated with primary antibodies overnight at 4◦C as follows: TSG101 (1:1000, Santa Cruz Biotechnology), HSP70 (1:1000, Santa Cruz Biotechnology), CD63 (1:1000, Santa Cruz Biotechnology), CD9 (1:1000, Santa Cruz Biotechnology), Cnr1 (1:1000, Santa Cruz Biotechnology), Rap1 (1:1000, Santa Cruz Biotechnology), neurofilament-M (NF-M) (1:1000, Proteintech Group), growth associated protein-43 (GAP43) (1:1000, Santa Cruz Biotechnology), myelinassociated glycoprotein (MAG) (1:1000, Proteintech Group), glial fibrillary acidic protein (GFAP) (1:1000, Proteintech Group), Bax (1:1000, Cell Signaling Technology), Bcl-2 (1:1000, Cell Signaling Technology), pro caspase-3 (1:1000, Cell Signaling Technology), cleaved-caspase-3 (1:1000, Cell Signaling Technology), total-PI3K (1:1000, Cell Signaling Technology), p-PI3K (1:1000, Cell Signaling Technology), total-AKT (1:1000, Cell Signaling Technology), and p-AKT (1:1000, Cell Signaling Technology).

Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot

Journal: Neuroscience letters

Article Title: Overexpression of miR-338-5p in exosomes derived from mesenchymal stromal cells provides neuroprotective effects by the Cnr1/Rap1/Akt pathway after spinal cord injury in rats.

doi: 10.1016/j.neulet.2021.136124

Figure Lengend Snippet: Fig. 4. Immunofluorescence double staining of NF-M, GAP43, MAG and GFAP levels following various treatments at day 4 post-SCI in rats. (A) Increased levels of the NF-M and GAP43 proteins in the region of anterior horn and adjacent lateral funiculus were observed in the miR-338-5p exo group compared to the other SCI groups at day 4 post-SCI. (B) Decreased protein levels of MAG and GFAP in the region of anterior horn and adjacent lateral funiculus were observed in the miR-338-5p exo group compared to the other SCI groups at day 4 post-SCI. Scale bar, 200 µm. (C) Semi-quantification of the mean fluorescence intensity of NF-M, GAP43, MAG and GFAP were measured. Data are presented as the mean ± SD. n = 3 per group, #p < 0.05, ##p < 0.01, ###p < 0.001, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After the membranes were blocked with 5% nonfat milk in TBST for 1 h at room temperature, they were incubated with primary antibodies overnight at 4◦C as follows: TSG101 (1:1000, Santa Cruz Biotechnology), HSP70 (1:1000, Santa Cruz Biotechnology), CD63 (1:1000, Santa Cruz Biotechnology), CD9 (1:1000, Santa Cruz Biotechnology), Cnr1 (1:1000, Santa Cruz Biotechnology), Rap1 (1:1000, Santa Cruz Biotechnology), neurofilament-M (NF-M) (1:1000, Proteintech Group), growth associated protein-43 (GAP43) (1:1000, Santa Cruz Biotechnology), myelinassociated glycoprotein (MAG) (1:1000, Proteintech Group), glial fibrillary acidic protein (GFAP) (1:1000, Proteintech Group), Bax (1:1000, Cell Signaling Technology), Bcl-2 (1:1000, Cell Signaling Technology), pro caspase-3 (1:1000, Cell Signaling Technology), cleaved-caspase-3 (1:1000, Cell Signaling Technology), total-PI3K (1:1000, Cell Signaling Technology), p-PI3K (1:1000, Cell Signaling Technology), total-AKT (1:1000, Cell Signaling Technology), and p-AKT (1:1000, Cell Signaling Technology).

Techniques: Immunofluorescence, Double Staining, Fluorescence

Journal: Neuroscience letters

Article Title: Overexpression of miR-338-5p in exosomes derived from mesenchymal stromal cells provides neuroprotective effects by the Cnr1/Rap1/Akt pathway after spinal cord injury in rats.

doi: 10.1016/j.neulet.2021.136124

Figure Lengend Snippet: Fig. 6. Overexpression of miR-338-5p alleviated H2O2-induced cell injury in PC12 cells. (A) PC12 cells were transfected with NC mimics/inhibitors, miR-338-5p mimics and inhibitors. qRT-PCR analysis of the miR-338-5p level. (B, C) Measurement of intracellular ROS and SOD. (D) CCK-8 assay for cell viability. (E, F) Western blot analysis of NF-M, GAP43, MAG and GFAP levels following various treatments in PC12 cells. n = 3 per group, #p < 0.05, ##p < 0.01, ###p < 0.001, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After the membranes were blocked with 5% nonfat milk in TBST for 1 h at room temperature, they were incubated with primary antibodies overnight at 4◦C as follows: TSG101 (1:1000, Santa Cruz Biotechnology), HSP70 (1:1000, Santa Cruz Biotechnology), CD63 (1:1000, Santa Cruz Biotechnology), CD9 (1:1000, Santa Cruz Biotechnology), Cnr1 (1:1000, Santa Cruz Biotechnology), Rap1 (1:1000, Santa Cruz Biotechnology), neurofilament-M (NF-M) (1:1000, Proteintech Group), growth associated protein-43 (GAP43) (1:1000, Santa Cruz Biotechnology), myelinassociated glycoprotein (MAG) (1:1000, Proteintech Group), glial fibrillary acidic protein (GFAP) (1:1000, Proteintech Group), Bax (1:1000, Cell Signaling Technology), Bcl-2 (1:1000, Cell Signaling Technology), pro caspase-3 (1:1000, Cell Signaling Technology), cleaved-caspase-3 (1:1000, Cell Signaling Technology), total-PI3K (1:1000, Cell Signaling Technology), p-PI3K (1:1000, Cell Signaling Technology), total-AKT (1:1000, Cell Signaling Technology), and p-AKT (1:1000, Cell Signaling Technology).

Techniques: Over Expression, Transfection, Quantitative RT-PCR, CCK-8 Assay, Western Blot

Journal: Scientific Reports

Article Title: Expression and regulation of FRMD6 in mouse DRG neurons and spinal cord after nerve injury

doi: 10.1038/s41598-020-58261-7

Figure Lengend Snippet: Primary antibodies.

Article Snippet: GAP43 , Rabbit , IHC (Coons) , 1 μg/ml , Atlas Antibodies AB/HPA015600.

Techniques:

Journal: Molecular Neurobiology

Article Title: Transplantation of MiR-28-5p-Modified BMSCs Promotes Functional Recovery After Spinal Cord Injury

doi: 10.1007/s12035-023-03702-3

Figure Lengend Snippet: Transplantation of lentivirus-mediated miR-28-5p-overexpressed BMSCs decreased the formation of glial scar and promoted axonal regeneration of SCI rats . A Co-immunofluorescent staining showed the glial scar (GFAP, red) and the axonal regeneration (GAP43, green) at 21 days after BMSC transplantation. B Quantification of GFAP immunoflurescent staining. C Quantification of GAP43 immunoflurescent staining. Data were presented with mean ± SD; # P < 0.05 vs. SCI group; & P < 0.05 vs. Lv-Con group

Article Snippet: Subsequently, tissues or cells were subjected to overnight incubation at 4 °C with primary antibodies, including microtubule-associated protein 2 (MAP2) at a dilution of 1:200 (Boster Biological Engineering Co.), neuron-specific enolase (NSE) at a dilution of 1:200 (Millipore), β3-tubulin at a dilution of 1:200 (Boster Biological Engineering Co.), neurofilament 200 (NF-200) at a dilution of 1:400 (CST), Notch1 at a dilution of 1:200 (Abcam), glial fibrillary acidic protein (GFAP) at a dilution of 1:600 (Boster Biological Engineering Co.), growth-associated protein 43 (GAP43) at a dilution of 1:200 (NOVUS), and myelin basic protein (MBP) at a dilution of 1:200 (Boster Biological Engineering Co.).

Techniques: Transplantation Assay, Staining

Journal: bioRxiv

Article Title: Adipose mTORC2 is essential for arborization of sensory neurons in white adipose tissue and whole-body energy homeostasis

doi: 10.1101/2022.03.21.485116

Figure Lengend Snippet: (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n=6;6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n=6;6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n=5;5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N=11;9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N=19;11).

Article Snippet: Primary antibodies used were RICTOR (1:1000; Cell signaling; Cat#2140), AKT (1:1000; Cell signaling, Cat#4685), AKT-pS473 (1:1000; Cell signaling, Cat#9271), CALNEXIN (1:1000, Enzo, Cat#ADI-SPA-860-F), GAP43 (1:1000, Cell signaling, Cat#8945), GAP43-pS41 (1:1000, R&D Systems, Cat#PPS006), Tyrosine hydroxylase (1:500, Millipore, Cat#AB1542), HSL (1:2000, Cell signaling, Cat#4107), HSL-pS660 (1:1000, Cell signaling, Cat#4126), HSL-pS563 (1:1000, Cell signaling, Cat#4139).

Techniques: Western Blot, Control

Journal: ESC Heart Failure

Article Title: The influence of inhibiting renal neural regeneration on the efficacy of renal denervation to chronic heart failure

doi: 10.1002/ehf2.13655

Figure Lengend Snippet: Comparisons of plasma parameters and mRNA expression of indicator of nerve regeneration in the renal arteries among the five groups. The levels of plasma renin (A), noradrenaline (B), Nog‐B (C), N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP) (D) and the ratio of heart to body weight (E) in the control, heart failure (HF), renal denervation (RDN), Nog and NEP groups. The relative mRNA levels of calcitonin gene‐related peptide (CGRP) (F), nerve growth factor (β‐NGF) (G), and growth‐associated protein 43 (GAP‐43) (H). * P < 0.05 vs. the HF and # P < 0.05 vs. the RDN group.

Article Snippet: After blocking with 5% non‐fat milk, membranes were incubated with the following primary antibodies: CGRP polyclonal antibody (1:8000; Sigma‐Aldrich, USA; Catalog C8198), β‐NGF (1:1000; R&D Systems Inc., USA; Catalog AF‐556‐NA), GAP‐43 (1:500; BIOSS, China; Catalog BS‐0154R), or β‐actin (1:1000; Boster, China; Catalog BM0627; Clone AC‐15).

Techniques: Expressing

Journal: ESC Heart Failure

Article Title: The influence of inhibiting renal neural regeneration on the efficacy of renal denervation to chronic heart failure

doi: 10.1002/ehf2.13655

Figure Lengend Snippet: The protein expression of calcitonin gene‐related peptide (CGRP), nerve growth factor (β‐NGF) and growth‐associated protein 43 (GAP‐43) in the renal arteries in the different groups. Western blot analysis of CGRP, GAP‐43 and β‐NGF expression in the control (A), heart failure (HF) (B), renal denervation (RDN) (C), Nog (D) and NEP (E) groups and relative mRNA levels of CGRP (F), β‐NGF (G), and GAP‐43 (H) in the renal arteries in the different groups. * P < 0.05 vs. the HF and # P < 0.05 vs. the RDN group.

Article Snippet: After blocking with 5% non‐fat milk, membranes were incubated with the following primary antibodies: CGRP polyclonal antibody (1:8000; Sigma‐Aldrich, USA; Catalog C8198), β‐NGF (1:1000; R&D Systems Inc., USA; Catalog AF‐556‐NA), GAP‐43 (1:500; BIOSS, China; Catalog BS‐0154R), or β‐actin (1:1000; Boster, China; Catalog BM0627; Clone AC‐15).

Techniques: Expressing, Western Blot